PMP™ Skin Microbiome Test
Not influenced by host DNA
RAPID
COST-EFFECTIVE
QUANTITATIVE
Fast-track your skin microbiome analysis with Bio-Me PMP™
Advantages of PMP™
- Not impacted by host DNA or low microbial biomass
- Accurate – sensitive, precise, reproducible
- Cost-effective
- Scalable – supports projects of any size
- Absolute quantification – measures abundance and microbial load
- High taxonomic resolution – subspecies level
- Comprehensive – more than 50 key skin microbes
- Fast – ready-to-use results in 2-5 days
- Simple data output – no bioinformatic steps needed
Bio-Me PMP™ technology – overcoming the challenges of host DNA
Skin microbiome samples are typically low in microbial biomass and heavily contaminated with human skin cells – in fact, over 90% of the total DNA in skin samples can correspond to human DNA. Shotgun metagenomics struggles in detecting microbial DNA in such samples reliably; high levels of host DNA impact detection rates and reproducibility and lead to high costs due to the higher sequencing depth needed. Amplicon sequencing, on the other hand, is not affected by host DNA but it provides poor taxonomic resolution and inaccurate abundance profiling. In contrast, the qPCR-based approach central to Bio-Me Precision Microbiome Profiling (PMP™) technology achieves subspecies resolution and is not negatively impacted by host DNA, because it is a targeted approach.
Bio-Me PMP™ – a superior solution for skin microbiome analysis
PMP™ technology is optimal for skin samples with low microbial biomass and high amounts of host DNA. Unlike sequencing, PMP™ can quantify the number of detected microorganisms in the sample. The PMP™ platform relies on highly parallelized qPCR, enabling the quantitative detection of many microbes simultaneously. All assays are carefully validated, highly specific and true-to-target, giving you accurate results. PMP™ technology has been demonstrated to return results concordant with class-leading shotgun sequencing approaches (manuscript in preparation), but at a fraction of the cost and with a shorter turnaround time (ready-to-use results are available in 2-5 days after samples arrive at the Bio-Me lab). DNA extraction has been optimized for robust skin microbes with tough cell walls and fully supports industry-standard skin sampling methods.
Who is the Bio-Me PMP™ Skin Microbiome Test for?
- Skincare and cosmetic product developers (‘microbiome-friendly’ claim substantiation)
- Nutrition and wellness professionals (Bio-Me PMP™ seamlessly integrates into your B2C business)
- Dermatology R&D
- Academic research
What does the Bio-Me PMP™ Skin Microbiome Test include?
The Bio-Me PMP™ Skin Microbiome Test v2.0 offers a highly comprehensive microbiome profiling solution targeting more than 50 rationally selected key bacterial and fungal skin species, to provide you with a meaningful picture of the skin microbiome, at a glance. The analysis of mites can be ordered as an add-on. Bio-Me offers combined gut and skin microbiome profiling for gut-skin axis studies. Custom qPCR assays and panel development are available upon request.
Comprehensive coverage of oily, dry and moist sites with subspecies resolution – without the costs, waiting times and data burden of sequencing.
